Spatial Distribution of Elements in the Brain of Rats Measured by Synchrotron Radiation X-ray Fluoresence

FENG Liu-Xing; XIAO Hai-Qing; HE Xiao; ZHANG Zhi-Yong; LIU Nian-Qing; ZHAO Yu-Liang; HUANG Yu-Ying

Chinese Physics C> 2005, Vol. 29> Issue(10) : 1012-1016

Spatial Distribution of Elements in the Brain of Rats Measured by Synchrotron Radiation X-ray Fluoresence

Key Laboratory of Nuclear Analytical Techniques,Institute of High Energy Physics,CAS,Beijing 100049,China2 Graduate School of the Chinese Academy of Sciences,Beijing 100049,China3 Beijing Synchrotron Radiation Facility,Institute of High Energy Physics,CAS,Beijing 100049,China

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Spatial(two-dimensional)distributions of inorganic elements such as chlorine,potassium, calcium,iron,copper,and zinc in the brain slices of Wistar rats were measured by synchrotron radiation X-ray fluorescence (SRXRF) analysis. Differences in two-dimensional distribution of these elements were observed. Chlorine, calcium, and zinc were primarily concentrated in hippocampus, while the potassium concentration was relatively higher in both cerebral cortex and hippocampus. However,the distribution of copper was comparatively ruleless in the three brain regions. The cluster analysis of the elemental results shows that the spatial distribution of chlorine was correlated well with the distribution of potassium.Since SRXRF is possible to obtain a pictorial representation of the elemental concentrations in tissue sections of brain, this nuclear method may be useful to evaluate the ionic changes in injured brain tissues in relation to histological observations.

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[1]

. Takahashi S, Takahashi I, Sato H et al. Biol. Trace Elem. Res., 2001, 80: 145—1582. Constantinidis J. Drug Dev. Res., 1992, 27: 1—143. Takahashi H, Tanaka S, Sano K. J Neurosurg, 1981, 55:708—7174. Atwood D, Yearwood B. J. Organomet. Chem., 2000, 600:186—1975. Sentaro T, Shizuo H, Yuichi T et al. Journal of Neuroscience Methods, 2000, 100: 53—626. Ehmann W D, Markesbcry W R, Hossain T I M. J. Radioanal. Chem., 1982, 70: 57—657. Paul M C, Parsonsb C H, Calfordb M B et al. Spectrochim Acta, 2004, B59: 1485—14908. Van Espen P, Janssens K, Nobels J. Chemometr. Intelligent Lab. Systems, 1987, 1: 109—1149. LIU N Q, LIU P S, WANG K J et al. Biol. Trace Elem. Res., 2000, 76: 279—28410. Paxinos G, Watson C. The Rat Brain in Stereotaxic Coordinates (3rd ed). San Diego: Academic Press, 1997. 28—6811. Van Espen P, Janssens K, Nobels J. Chemometr. Intelligent Lab. Systems, 1987, 1: 109—11412. Vekemans B, Janssens K, Vincze L et al. X-Ray Spectrometry, 1994, 23: 278—28513. Hansen A J. Physiol Rev., 1985, 65: 101—14814. Takeda A. Yakugaku Zasshi., 2004, 124: 577—58515. Batra J, Seth P K. Indian. J. Clin. Biochem., 2002, 17:108—114

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FENG Liu-Xing, XIAO Hai-Qing, HE Xiao, ZHANG Zhi-Yong, LIU Nian-Qing, ZHAO Yu-Liang and HUANG Yu-Ying. Spatial Distribution of Elements in the Brain of Rats Measured by Synchrotron Radiation X-ray Fluoresence[J]. Chinese Physics C, 2005, 29(10): 1012-1016.

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Received: 2005-02-17
Revised: 1900-01-01

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

Spatial Distribution of Elements in the Brain of Rats Measured by Synchrotron Radiation X-ray Fluoresence

Corresponding author: ZHANG Zhi-Yong,

Key Laboratory of Nuclear Analytical Techniques,Institute of High Energy Physics,CAS,Beijing 100049,China2 Graduate School of the Chinese Academy of Sciences,Beijing 100049,China3 Beijing Synchrotron Radiation Facility,Institute of High Energy Physics,CAS,Beijing 100049,China

Received Date: 2005-02-17

Accepted Date: 1900-01-01

Available Online: 2005-10-05

Abstract: Spatial(two-dimensional)distributions of inorganic elements such as chlorine,potassium, calcium,iron,copper,and zinc in the brain slices of Wistar rats were measured by synchrotron radiation X-ray fluorescence (SRXRF) analysis. Differences in two-dimensional distribution of these elements were observed. Chlorine, calcium, and zinc were primarily concentrated in hippocampus, while the potassium concentration was relatively higher in both cerebral cortex and hippocampus. However,the distribution of copper was comparatively ruleless in the three brain regions. The cluster analysis of the elemental results shows that the spatial distribution of chlorine was correlated well with the distribution of potassium.Since SRXRF is possible to obtain a pictorial representation of the elemental concentrations in tissue sections of brain, this nuclear method may be useful to evaluate the ionic changes in injured brain tissues in relation to histological observations.

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Spatial Distribution of Elements in the Brain of Rats Measured by Synchrotron Radiation X-ray Fluoresence

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