Small Angle X-ray Scattering Study of the Influence of Temperature on the Structure of DNA/Lipid Complexes

HU Shu-Xin; LI Da-Peng; MAI Zhen-Hong; LI Ming

Chinese Physics C> 2005, Vol. 29> Issue(S1) : 4-7

Small Angle X-ray Scattering Study of the Influence of Temperature on the Structure of DNA/Lipid Complexes

中国科学院高能物理研究所

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Abstract：
We report on the synchrotron small-angle X-ray scattering (SAXS) study of self-assembled complex formed by mixing short-chain DNAs with cationic phospholipids in water. The DNA/lipid complex is a sandwiched structure, consisting of alternate layers of DNAs and lipid bilayers with a periodicity of d_D/L. The DNAs at the interfaces are one-dimensionally ordered with a periodicity of d_DNA. When the temperature of the system is increased, the lipid bilayers become soft so that the DNAs are trapped more deeply inside the lipid bilayers. This explains why the measured d_D/L decreases insignificantly with the increase of temperature.On the other hand, the value of d_DNA keeps constant because of the stiffness of the DNAs.

References

[1]

. Chesony S, Huang L. Annu. Rev. Biophys. Biomol. Strut.,2000, 29: 272. Safinya Cyrus R, Curr. Opin. Strut. Biol., 2001, 11: 4403. R#228;dler Joachim O, Koltover Ilya, Salditt Tim et al. Science,1997, 275: 8104. Koltover Ilya, Sahu Sanjukta, Davis Nicolynn. Angew.Chem. Int. Ed., 2004, 43: 40345. Salditt Tim, Koltover Ilya, R#228;dler Joachim O. Phys. Rev.,1998, E58: 8896. Natali F, Castellano C, Pozzi D et al. Biophys. J., 2005,88(2): 10817. Caracciolo G, Caminiti R, Pozzi D et al. Chem. Phys. Lett.,2002, 351: 2228. Weisman S, Hirsch-Lerner D, Barenholz Y et al. Biophys.J., 2004, 87: 6099. Koltover I, Salditt T, Safinya C R. Biophys. J., 1999, 77:915

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HU Shu-Xin, LI Da-Peng, MAI Zhen-Hong and LI Ming. Small Angle X-ray Scattering Study of the Influence of Temperature on the Structure of DNA/Lipid Complexes[J]. Chinese Physics C, 2005, 29(S1): 4-7.

HU Shu-Xin, LI Da-Peng, MAI Zhen-Hong and LI Ming. Small Angle X-ray Scattering Study of the Influence of Temperature on the Structure of DNA/Lipid Complexes[J]. Chinese Physics C, 2005, 29(S1): 4-7. shu

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Received: 2005-10-28
Revised: 1900-01-01

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

Small Angle X-ray Scattering Study of the Influence of Temperature on the Structure of DNA/Lipid Complexes

Corresponding author: LI Ming,

中国科学院高能物理研究所

Received Date: 2005-10-28

Accepted Date: 1900-01-01

Available Online: 2005-01-02

Abstract: We report on the synchrotron small-angle X-ray scattering (SAXS) study of self-assembled complex formed by mixing short-chain DNAs with cationic phospholipids in water. The DNA/lipid complex is a sandwiched structure, consisting of alternate layers of DNAs and lipid bilayers with a periodicity of d_D/L. The DNAs at the interfaces are one-dimensionally ordered with a periodicity of d_DNA. When the temperature of the system is increased, the lipid bilayers become soft so that the DNAs are trapped more deeply inside the lipid bilayers. This explains why the measured d_D/L decreases insignificantly with the increase of temperature.On the other hand, the value of d_DNA keeps constant because of the stiffness of the DNAs.

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Small Angle X-ray Scattering Study of the Influence of Temperature on the Structure of DNA/Lipid Complexes

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