×
近期发现有不法分子冒充我刊与作者联系,借此进行欺诈等不法行为,请广大作者加以鉴别,如遇诈骗行为,请第一时间与我刊编辑部联系确认(《中国物理C》(英文)编辑部电话:010-88235947,010-88236950),并作报警处理。
本刊再次郑重声明:
(1)本刊官方网址为cpc.ihep.ac.cn和https://iopscience.iop.org/journal/1674-1137
(2)本刊采编系统作者中心是投稿的唯一路径,该系统为ScholarOne远程稿件采编系统,仅在本刊投稿网网址(https://mc03.manuscriptcentral.com/cpc)设有登录入口。本刊不接受其他方式的投稿,如打印稿投稿、E-mail信箱投稿等,若以此种方式接收投稿均为假冒。
(3)所有投稿均需经过严格的同行评议、编辑加工后方可发表,本刊不存在所谓的“编辑部内部征稿”。如果有人以“编辑部内部人员”名义帮助作者发稿,并收取发表费用,均为假冒。
                  
《中国物理C》(英文)编辑部
2024年10月30日

MXAN Analysis of the XANES Energy Region of LiPDF

  • Leptospira interrogans peptide deformylase (LiPDF) is an important Zinc metalloprotein and plays a key role in protein synthesis of Leptospira interrogans, an ubiquitous environmental bacterium that can cause the infections of animals and humans. It is an attractive target for the design of new antibiotics since the deformyling apparently does not occur in the cytoplasm of eucaryotic cells. In this paper, we probed the protein in pH3.0 solution by X-ray absorption near-edge structure (XANES) spectroscopy. We quantitatively determined the local structure around the metal center using MXAN procedure, which is based on Multiple Scattering theory. The experimental XANES spectrum of LiPDF has been reconstructed perfectly using suitable initial structure mode, and the corresponding local structure of the active center provides structure information for understanding the pH-dependent enzymatic activity.
  • 加载中
  • [1] . Hasnain S S, Hodgson K O. J. Synchrotron Rad., 1999, 6:8522. Koningsberger D C, Prins R. X-Ray Absorption: Princi-ples, Applications, Techniques of EXAFS, SEXAFS and XANES. New York: John Wiley Sons Inc., 1988. 257-3203. Scott R A. Structural and Resonance Techniques in Biological Research. Rousseau D L, ed., Orlando, Florida:Academic Press, 1984. 2954. Powers L. Biochim. Biophys. Acta, 1982, 1: 6835. Rehr J J, Ankudinov A L. J. Synchrotron Rad., 2003, 10:436. Natoli C R, Benfatto M, Della Longa S et al. J. SynchrotronRad., 2003, 10: 267. Natoli C R, Benfatto M. A Unifying Scheme of Interpretation of X-Ray Absorption Spectra Based on the Multiple Scattering Theory. In: Lagarde P, Raoux D, Petiau J. EXAFS and Near Edge Structure Ⅳ, Volume 1. Paris: Les Editions de Physique, 1986. 11-238. Della Longa S, Arcovito A, Benfatto M et al. Physical Review Letters, 2001, 87: 155501(1-4)9. Benfatto M, Congiu-Castellano A, Della Longa S et al. J.Synchrotron Rad., 2001, 8: 267-26910. Benfattoa M, Della Longa S. J. Synchrotron Rad., 2001, 8:1087-109411. Benfatto M, Della Long S, Natoli C R. J. Synchrotron Rad.,2003, 10: 51-5712. Durham P, Pendry J B, Hodges C H. Comput. Phys. Commun., 1982, 25: 193-20013. Norman J G. Mol. Phys., 1976, 31: 1191-119814. Tyson T A, Hodgson K O, Benfatto M. Phys. Rev., 1992,B46: 5997-601915. Hedin L, Lundqvist S. Solid State Phys., 1969, 23: 1-18116. Benfatto M, Solera J A. Garcia J. Phys. Rev., 1997, B56:2447-245217. Rehr J J, Albers R C. Rev. Mod., Phys., 2000, 72: 621-65418. LI Y, REN S, GONG W. Acta Cryst. D., 2002, 58: 846-84819. LI Shu-Jun, ZHOU Zhao-Cai, WU Zi-Yu et al. J. Synchrotron Rad., 2005, 12: 111-11420. LI Y, CHEN Z, GONG W. Biochem Biophys Res Commun., 2002, 295(4): 884-889
  • 加载中

Get Citation
GUO Xiao-Yun, CHU Wang-Sheng, GONG Wei-Min, DONG Yu-Hui, XIE Ya-Ning, YANG Fei-Fei, Maurizio Benfatto and WU Zi-Yu. MXAN Analysis of the XANES Energy Region of LiPDF[J]. Chinese Physics C, 2007, 31(2): 199-203.
GUO Xiao-Yun, CHU Wang-Sheng, GONG Wei-Min, DONG Yu-Hui, XIE Ya-Ning, YANG Fei-Fei, Maurizio Benfatto and WU Zi-Yu. MXAN Analysis of the XANES Energy Region of LiPDF[J]. Chinese Physics C, 2007, 31(2): 199-203. shu
Milestone
Received: 2006-05-17
Revised: 2006-05-23
Article Metric

Article Views(4316)
PDF Downloads(647)
Cited by(0)
Policy on re-use
To reuse of subscription content published by CPC, the users need to request permission from CPC, unless the content was published under an Open Access license which automatically permits that type of reuse.
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Email This Article

Title:
Email:

MXAN Analysis of the XANES Energy Region of LiPDF

    Corresponding author: Maurizio Benfatto,
  • School of Life Science,University of Science and Technology of China, Hefei 230026, China2 Institute of High Energy Physics, CAS, Beijing 100049, China3 National Laboratory of Biomacromolecules, Institute of Biophysics,Chinese Academy of Sciences, Beijing 100101, China4 Instituto Nazionale di Fisica Nucleare, Laboratori Nazionali di Frascati, P.O. Box 13, 00044, Frascati, Italy

Abstract: Leptospira interrogans peptide deformylase (LiPDF) is an important Zinc metalloprotein and plays a key role in protein synthesis of Leptospira interrogans, an ubiquitous environmental bacterium that can cause the infections of animals and humans. It is an attractive target for the design of new antibiotics since the deformyling apparently does not occur in the cytoplasm of eucaryotic cells. In this paper, we probed the protein in pH3.0 solution by X-ray absorption near-edge structure (XANES) spectroscopy. We quantitatively determined the local structure around the metal center using MXAN procedure, which is based on Multiple Scattering theory. The experimental XANES spectrum of LiPDF has been reconstructed perfectly using suitable initial structure mode, and the corresponding local structure of the active center provides structure information for understanding the pH-dependent enzymatic activity.

    HTML

Reference (1)

目录

/

DownLoad:  Full-Size Img  PowerPoint
Return
Return