MXAN Analysis of the XANES Energy Region of LiPDF
- Received Date: 2006-05-17
- Accepted Date: 2006-05-23
- Available Online: 2007-02-05
Abstract: Leptospira interrogans peptide deformylase (LiPDF) is an important Zinc metalloprotein and plays a key role in protein synthesis of Leptospira interrogans, an ubiquitous environmental bacterium that can cause the infections of animals and humans. It is an attractive target for the design of new antibiotics since the deformyling apparently does not occur in the cytoplasm of eucaryotic cells. In this paper, we probed the protein in pH3.0 solution by X-ray absorption near-edge structure (XANES) spectroscopy. We quantitatively determined the local structure around the metal center using MXAN procedure, which is based on Multiple Scattering theory. The experimental XANES spectrum of LiPDF has been reconstructed perfectly using suitable initial structure mode, and the corresponding local structure of the active center provides structure information for understanding the pH-dependent enzymatic activity.