Study of the Effect of Urea on the Phase Transition of Phospholipid by Synchrotron X-Ray Diffraction

YU Zhi-Wu; FENG Ying; WANG Jun; DONG Bao-Zhong

Chinese Physics C> 2001, Vol. 25> Issue(S1) : 75-80

Study of the Effect of Urea on the Phase Transition of Phospholipid by Synchrotron X-Ray Diffraction

Department of Chemistry,Tsinghua University,Beijing 100084,China2 Institute of High Energy Physics,CAS,Beijing 100039,China3 Division of Life Sciences,King's College London,150 Stanford St.,LondonSEI 8WA,UK

Abstract
HTML
Reference
Related

PDF

Abstract：
Synchrotron X-ray diffraction and differential scanning calorimetry were used to study the effect of urea solutions on the phase behavior of Dioleoylglycero-phosphatidvlethanolamine (DOPE).It was found that urea could stabilize lamellar liquid-crystalline phase at the expense of lamellar-gel phase and non-bilayer inverted hexagonal phase.The repeat distance of DOPE multilamellar structure increases with the increasing of urea concentration.The molecular mechanisms are discussed in terms of the possible hydrogen bonds between urea and the phospholipid head groups and the Hofmeister effect of urea,where urea is taken as a waterstructure breaker.In addition,X-ray diffraction pattern of a wet rat tail collagen fiber was collected and was used as calibration standard at small-angle diffraction stations.

References

[1]

YANG Fu-Yu, HUANG Fen. Membrane Lipid-Membrane Protein Interaction and Application in Medicine and AgricultureJi'nan: Science and Technique Publisher of Shandong, 1996. 140-141 (in Chinese)(杨福愉，黄芬.膜脂一膜蛋白相互作用及其在医学和农业上的应用.济南:山东科学技术出版社，1996. 140-141)2 Shalaev E Y, Steponkus P L. Biochim. Biophys. Acts, 1999,1419:229-2473 ZHANG Zhi-Hong, LIU Weng-Long. Membrane Biological Physics. Beijing: High Education Press of China, 1987. 44 (inChinese)(张志鸿，刘文龙.膜生物物理学.北京:高等教育出版社，1987. 44)4 Koynova R, Caffrey M. Chem. Phys. Lipids, 1994, 69: 1-345 YU Z W, Quinn P J. Mol. Membr. Biol., 1998, 15:59-68.6 Seddon J M. Biochim. Biophys. Acta, 1990, 1031:1-697 Brennan J D, Kallury K MR, Krull U J. Thin Solid Films, 1994, 244:898-9048 Surrey T, Jahnig F. J. Biol. Chem., 1995, 270:28199-282039 Carlson S R, Goldstein L. J. Exp. Zool., 1997, 277:275-28210 Patra S K, Alonso A, Go}ii F M. Biochim. Biophys. Acta, 1998, 1373:112-I 1811 Bentley M V L B, Kedor E R M, Vianna R F et al. Int. J. Pharm., 1997, 146:255-26212 Sanderson P W, Lis L J, Quinn P J et al. Biochim. Biophys. Acta, 1991, 1067:43-5013 Bergenstahl B A, Stenius P. J. Phys. Chem., 1987, 91:5944-5948.14 Gicquaud C. Auger M, Wong P T T et al. Arch. Biochem. Biophys, 1996, 334:193-19915 Li S J, Kinoshita K, Furuike S et al. Biophys. Chem., 1999, 81:191-19616 Barton K N, Buhr M M. Ballantyne J S. Am. J. Physiol., 1999, 276:8397-840617 Huxley H E, Brown W. J. Mol. Biol., 1967, 30:383-43418 YU Z W, Quinn P J. Biochim. Biophys. Acta., 2000, 1509:440-450.19 YU Z W, Williams W P, Quinn P J. Arch. Biochem. Biophys.. 1996, 332:187-19520 Soloaga A, Ramirez J M, Goni F M. Biochemistry, 1998, 37:6387-639321Yeagle P L, Sen A Tovchigrechko A, Biochemistry, 1986, 25:7518-752222 Rodnikova M, Barthel J. J. Mol. Liq., 1999, 79:187-20123 Kinoshita K, Yamazaki M. Biochim. Biophys. Acta., 1997, 1330:199-206

[1]	SUN Bao-Gen , WANG Bao-Yun , WANG Ji-Gang , WANG Jun-Hua , LU Ping , SHEN Chao-Bo . Research and Measurement of Synchrotron Radiation System for HLS. Chinese Physics C, 2008, 32(S1): 133-135.
[2]	SUN Tian-Xi , XIE Ya-Ning , LIU Zhi-Guo , LIU Tao , HU Tian-Dou , YANG Jun , CHU Xue-Lian , DING Xun-Liang . Application of Monolithic Polycapillary X-Ray Lens in Focusing of Synchrotron Radiation. Chinese Physics C, 2005, 29(S1): 137-139.
[3]	YANG Kai-Feng , CUI Qi-Liang , MA Yan-Mei , ZHAO Yu , LI Bing , WANG Yi , ZHOU Qiang , LUO Cong-Ju , LIU Jing , ZOU Guang-Tian . Synchrotron Radiation Diffraction of Tellurium Under High Pressure And High Temperature. Chinese Physics C, 2005, 29(S1): 123-125.
[4]	CHEN Zhong-Jun , WU Zong-Hua , SUN Ming-Hua , CHEN Xing , CAI Quan , YANG Shi-Shun . Imaging Plate Powder Diffraction System for Synchrotron Radiation. Chinese Physics C, 2005, 29(3): 321-324.
[5]	YUAN Qing-Xi , WANG Jun-Yue , ZHU Pei-Ping , HUANG Wan-Xia , SHU Hang , HU Tian-Dou , WU Ziyu . Computed Tomography Using Peak-Position Image of Diffraction Enhanced Imaging. Chinese Physics C, 2005, 29(10): 1023-1026.
[6]	HU Shu-Xin , LI Da-Peng , MAI Zhen-Hong , LI Ming . Small Angle X-ray Scattering Study of the Influence of Temperature on the Structure of DNA/Lipid Complexes. Chinese Physics C, 2005, 29(S1): 4-7.
[7]	SHEN Da-Wa , YU Hui , WU Zong-Hua , HUANG Yong . The Morphology of CDA-g-PMMA Copolymers in Solution Studied by SR-SAXS. Chinese Physics C, 2005, 29(S1): 8-11.
[8]	ZHOU Chun-Lan , WANG Dan-Ni , HE Chuang-Xin , WANG Bao-Yi , WEI Long , HE Qing , JIA Quan-Jie . Study on Pores and Defect by Synthrotron Radiation Small Angle Reflection Method and Slow Positron Annihilation Technique. Chinese Physics C, 2003, 27(S1): 67-71.
[9]	ZHENG Lei , CUI Ming-Qi , ZHAO Yi-Dong , ZHU Jie , ZHU Pei-Ping . Research of Photoionization Cross-Section of Argon in Soft X-Ray Region of Synchrotron Radiation. Chinese Physics C, 2003, 27(6): 555-558.
[10]	LIU Yi , DONG Bao-Zhong , WANG Jun , DING Yong-Fan , WU Zhong-Hua , LI Zhi-Hong , LI Kai-Xi , WU Dong . The Establishment of a Synchrotron Radiation Two Dimensional Small Angle X-Ray Scattering Equipment and It′s Application. Chinese Physics C, 2002, 26(1): 87-92.
[11]	ZHANG Wei-Wei , CUI Qi-Liang , PANG Yue-Wu , ZHU Pin-Wen , MA Hong-An , ZOU Guang-Tian , LIU Jing , LI Yan-Chun , LI Xiao-Dong . EDXD Study of α-LiIO₃ under High Pressure. Chinese Physics C, 2001, 25(S1): 62-65.
[12]	XU Ya-Bo , LI Hong-Nian , BAO Shi-Ning , LI Hai-Yang , QIAN Hai-Jie , LIU Feng-Qin , Kurash Ibrahim , JIA Jin-Feng , XUE Qi-Kun . Synchrotron Radiation Angle-Resolved Photoemission Study of the Orientational Phase Transition for K₃C₆₀. Chinese Physics C, 2001, 25(S1): 33-38.
[13]	ZHENG Wen-Li , JIA Quan-Jie , JIANG Xiao-Ming , JIANG Zui-Min . Study of Ge Thin Heterostructures by Synchrotron Radiation X-Ray Reflection. Chinese Physics C, 2001, 25(12): 1231-1237.
[14]	JIANG XiaoMing , JIA QuanJie , ZHENG WenLi , LIU Peng , XIAN DingChang , JIANG ZuiMin , WANG Xun . Development of Synchrotron Radiation X-Ray Grazing Incident Diffraction Method. Chinese Physics C, 2000, 24(12): 1185-1190.
[15]	Jiang Jianhua , Wang Zhouguang , Tian Yulian , Wang Gongli , Zhao Yang , Cao Mang , Li Dacheng , , . Study of X-Ray Interference Using Synchrotron Radiation. Chinese Physics C, 1998, 22(8): 763-767.
[16]	Wang Dewu , Liu Jianfei . Application of BSRF X-Ray Diffraction Station to Researches in Material Science. Chinese Physics C, 1995, 19(5): 473-478.
[17]	Dong Baozhong , Sheng Wenjun , Li Guangcheng , Zhang Zhijie . Small Angle Scattering Station of BEPC Synchrotron Radiation Laboratory. Chinese Physics C, 1995, 19(3): 284-288.
[18]	Wang Jun , Cui Mingqi , Xu Wenxuan , Huang Yuying , Wang Dewu , Liu Jianfei , Xian Dingchang . Study on Structure of Multilayers by Synchrotron Radiation Small Angle Diffraction Method. Chinese Physics C, 1994, 18(1): 13-19.
[19]	ZHAO Ji-Yong , YANG Ping , JIANG Jian-Hua , TIAN Yu-Lian , HAN Yong , WANG Chun-Xi , SHI Cai-Tu , XIAN Ding-Chang . Light Source for Synchrotron Radiation X-Ray Topography Study at Beijing Syncrotron Radiation Laboratory (BSRL). Chinese Physics C, 1992, 16(8): 679-684.
[20]	CAO Zhong , LIU Wei-Ren , WANG Li-Lie , XIE Shi-Liang , JIANG Li-Ping , DING Mei-Song , ZHAO Ji-Lai , REN You-Lai , SHAN Bing , ZHAO Zheng , CUI Ming-Qi . SHAPE MEASUREMENT OF SYNCHROTRON RADIATION WITH HIGH TIME RESOLUTION. Chinese Physics C, 1990, 14(8): 677-683.

Access

Get Citation

YU Zhi-Wu, FENG Ying, WANG Jun and DONG Bao-Zhong. Study of the Effect of Urea on the Phase Transition of Phospholipid by Synchrotron X-Ray Diffraction[J]. Chinese Physics C, 2001, 25(S1): 75-80.

YU Zhi-Wu, FENG Ying, WANG Jun and DONG Bao-Zhong. Study of the Effect of Urea on the Phase Transition of Phospholipid by Synchrotron X-Ray Diffraction[J]. Chinese Physics C, 2001, 25(S1): 75-80. shu

RIS(for EndNote,Reference Manager,ProCite)

BibTex

Txt

Milestone

Received: 1900-01-01
Revised: 1900-01-01

Article Metric

Article Views(3615)
PDF Downloads(546)
Cited by(0)

Policy on re-use

To reuse of subscription content published by CPC, the users need to request permission from CPC, unless the content was published under an Open Access license which automatically permits that type of reuse.

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Study of the Effect of Urea on the Phase Transition of Phospholipid by Synchrotron X-Ray Diffraction

Corresponding author: YU Zhi-Wu,

Department of Chemistry,Tsinghua University,Beijing 100084,China2 Institute of High Energy Physics,CAS,Beijing 100039,China3 Division of Life Sciences,King's College London,150 Stanford St.,LondonSEI 8WA,UK

Received Date: 1900-01-01

Accepted Date: 1900-01-01

Available Online: 2001-06-22

Abstract: Synchrotron X-ray diffraction and differential scanning calorimetry were used to study the effect of urea solutions on the phase behavior of Dioleoylglycero-phosphatidvlethanolamine (DOPE).It was found that urea could stabilize lamellar liquid-crystalline phase at the expense of lamellar-gel phase and non-bilayer inverted hexagonal phase.The repeat distance of DOPE multilamellar structure increases with the increasing of urea concentration.The molecular mechanisms are discussed in terms of the possible hydrogen bonds between urea and the phospholipid head groups and the Hofmeister effect of urea,where urea is taken as a waterstructure breaker.In addition,X-ray diffraction pattern of a wet rat tail collagen fiber was collected and was used as calibration standard at small-angle diffraction stations.

HTML

Reference (1)

Study of the Effect of Urea on the Phase Transition of Phospholipid by Synchrotron X-Ray Diffraction

Abstract：

References

Access

Article Metrics

Metrics

通讯作者: 陈斌, bchen63@163.com

Email This Article