Studies on Bacillus substilis DNA Lesions Induced by Fast Neutron Radiation

  • The content of Bacillus substilis var. niger (ATCC 9372) DNA double-strand breaks induced by neutron radiation at different doses and different dose rates respectively, was measured and quantified by pulsed-field gel electrophoresis. By the analyses of DNA release percentage (PR), DNA breakage level (L), average molecular weight of breakage DNA and DNA fragment distribution, the results showed that DNA fragments were obviously distributed into two regions in the different irradiation condition. This suggestes that there are some sensitive points to neutron radiation in the Bacillus substilis var. niger molecule. The DNA release percentage, DNA breakage level and the content of DNA fragments in different region varied regularly in some extent with change of the neutron radiation dose or dose rate.
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  • [1] . XIA Shou-Xuan. Radiobiology. Beijing: The Academy of Military Medical Sciences Press, 1998. 190-198(in Chi-nese) (夏寿萱主编.放射生物学.北京:军事医学科学出版社,1998.190-198)2. Pinto M, Prise K M, Michael B D. Int. J Radiat Biol., 2002,78(5): 375-3883. ZHENG Chun, LI Jian-Sheng, WU Jian-Hua. Nuclear Electronics Detection Technology, 2001, 21(4): 307-309(in Chi-nese) (郑春,李建胜,吴建华.核电子学与探测技术,2001, 21(4):307-309)4. ZANG Li-Hui, WEI Zhi-Yong, LI Ming et al. Chin. JHemorh, 2005, 15(2): 193-196(in Chi-nese) (臧黎慧,魏志勇,李明等.中国血液流变学杂志,2005, 15(2):193-196)5. ZHOU Guang-Ming, LI Wen-Jian, WANG Ju-Fang et al.Journal of Radiation Research and Radiation Processing,2001, 19(4): 289-294(in Chi-nese) (周光明,李文建,王菊芳等.辐射研究与辐射工艺学报,200119(4): 289-294)6. SUI Li, ZHAO Kui, NI Mei-Nan et al. HEP NP, 2004,28(10): 1126-1130(in Chi-nese)(隋丽,赵葵,倪嵋楠等.高能物理与核物理,2004, 28(10)1126-1130) 7. Nikjoo H, Nell P O, Wilson W E et al. Radiation Research,2001, 156: 577-5838. Rydberg B. Radiation Research, 1996, 145: 200-2099. Koufen P, Brdiczka D, Stark G. Int. J. Radiat. Biol., 2000,76(5): 625-63110. WEI Zeng-Quan, ZHOU Guang-Ming, WANG Ju-Fang etal. In: Book of Abstracts in 6th Workshop on Heavy-Charged Particles in Biology and Medicine. Carbon IonInduced DNA Double-Strand Breaks in Melanophore B16.Baveno, Italy. 1997. 711. Sutherland B M, Bennett P V, Sidorkina O et al. Biochemistry,2000, 39(27): 8026-803112. Newman H C, Prise K M, Fclkard M et al. Int. J. Radiat.Biol., 1997, 71(4): 347-36313. Lobrich M, Cooper P K, Rydberg B. Int. J. Radiat. Biol.,1996, 70(2): 493-50314. ZHOU Guang-Ming, LI Wen-Jian, WANG Ju-Fang et al.Acta Biophysica Sinica, 2000, 16(1): 139-145(in Chi-nese)(周光明,李文建,王菊芳等.生物物理学报,2000, 16(1):139-145)15. ZHOU Guang-Ming, LI Wen-Jian, Gao Qing-Xiang et al.Nuclear Physics Review, 2002, 20(1): 52-54(in Chi-nese)(周光明,李文建,高清祥等.原子核物理评论,2002, 20(1):52-54) 16. Schmid E, Regulla D, Guldbakke S et al. Radiation Research,2002, 157: 453-46017. Hacker U B, Kohnlein W, Kronholz H L et al. RadiationResearch, 2000, 153(6): 734-74218. Lowy R J, Vavrina G A, Labarre D D. Antiviral Research,2001, 52: 261-273
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CHEN Xiao-Ming, TAN Bi-Sheng, ZHENG Chun, ZHANG Jian-Guo, WANG Dan, CHU Shi-Jin and REN Zheng-Long. Studies on Bacillus substilis DNA Lesions Induced by Fast Neutron Radiation[J]. Chinese Physics C, 2007, 31(10): 972-977.
CHEN Xiao-Ming, TAN Bi-Sheng, ZHENG Chun, ZHANG Jian-Guo, WANG Dan, CHU Shi-Jin and REN Zheng-Long. Studies on Bacillus substilis DNA Lesions Induced by Fast Neutron Radiation[J]. Chinese Physics C, 2007, 31(10): 972-977. shu
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Received: 2006-10-19
Revised: 2007-03-22
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Studies on Bacillus substilis DNA Lesions Induced by Fast Neutron Radiation

    Corresponding author: REN Zheng-Long,
  • Southwest University of Science and Technology, Mianyang 621010, China2 Sichuan Agriculture University, Yaan 625000, China3 Institute of Nuclear Physics and Chemistry, China Academy of Engineering Physics, Mianyang 621900, China

Abstract: The content of Bacillus substilis var. niger (ATCC 9372) DNA double-strand breaks induced by neutron radiation at different doses and different dose rates respectively, was measured and quantified by pulsed-field gel electrophoresis. By the analyses of DNA release percentage (PR), DNA breakage level (L), average molecular weight of breakage DNA and DNA fragment distribution, the results showed that DNA fragments were obviously distributed into two regions in the different irradiation condition. This suggestes that there are some sensitive points to neutron radiation in the Bacillus substilis var. niger molecule. The DNA release percentage, DNA breakage level and the content of DNA fragments in different region varied regularly in some extent with change of the neutron radiation dose or dose rate.

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