Influence of 12C6+ ion irradiation on mutant avermitilis

  • The effects of 12C+6 ion irradiation on colony morphology and mycelia morphology, as well as on mutation rate have been studied in the B1a high-product strains (ZJAV-Y1-203) mutated by heavy ion irradiation and compared with that in the original strain (ZJAV-A-1). After irradiating the rate of a straw hat colony type having a high ability of producing B1a in ZJAV-Y1-203 strains was higher than that found in ZJAV-A-1 strains. When strains were cultured in a liquid medium for 24 hours, the mycelium becoming thinner could be observed in all of the irradiated ZJAV- Y1-203 groups, but only in the ZJAV-A-1 groups irradiated at the dose of 50 Gy or more. The early growth of mycelium was inhibited in the ZJAV- Y1-203 group irradiated with a high dose. The highest positive mutation rate (23.5%) of ZJAV - Y1 - 203 was reached at the lower dose of 30 Gy while the highest positive mutation rate of 34.2% in ZJAV-A-1 appeared at 50 Gy.
    These results indicate that the effects of heavy ion irradiation still exist even in the mutated Streptomyces avermitilis, and only the dose is lower and the effects not so strong compared with the one that is first irradiated with optimized heavy ion doses. This is evidence of the one directional mutation being controlled by many more factors in a organism.
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  • [1] LIU F, FU W, YAN H. Chinese Journal of Antibiotics, 2003, 28(9): 517-520[2] WU F J. Mutagenesis and Screening of Streptomyces Avermitilis Producing Avermitilis and the Fermentation Condition Optimization (Master Thesis). Lanzhou: Gansu Agricultural University, 2007, 6[3] LI R M, WANG J F, LI W J. Nuclear Physics Review, 2007, 24(3): 234-237[4] WANG S Y, CHEN J H, LI W J et al. IMP HIRFL Annual Report, 2008, 85-86[5] WANG S Y, BO Y H, CHEN J H et al. RAPD IMP HIRFL Annual Report, 2009, 124[6] WANG S Y, CHEN J H, LI W J et al. Nuclear Physics Review, 2010, 20(1): 82-87[7] CHEN J H, WANG S Y, LIU J et al. Journal of Gansu Agricultural University, 2010, 45(3): 85-87[8] Egerton J R, Ostlind D A, Blair L S et al. Agents Chemother, 1979, 15: 372-378[9] Ikeda H, mura S J. Antibiot, 1995, 48: 548-562. (e) R.D.[10] Boxall A B, Fogg L A, Kay P et al. Toxicology Letters, 2003a. 142: 207-218[11] Boxall A B, Kolpin D W, Halling-Sorensen B et al. Environmental Science and Technology, 2003, 37: 286A-294A[12] Floate K D, Wardhaugh K G, Boxall A B et al. Annual Review of Entomology, 2005, 50: 153-179[13] Kvecses J, Marcogliese D J. Scientific and Technical Report ST-233E. Environment Canada e Quebec Region, Environmental Conservation, St. Lawrence Centre, 2005, 72[14] Burg R W, Miller B M, Baker E E et al. Agents Chemother, 1979, 15: 361-367[15] FENG Z Y, WANG L Y, Rajski S R et al. Gan. Med. Chen., 2009, 17(6): 2147-2153[16] Mandell G L, Bennet J E, Dolin R. (Eds.) Principles and Practice of Infectious Diseases. 5th ed. Philadelphia: Churchill Livingstone. 2000, 205[17] Guzzo C A, Clineschmidt C M, Schorn J M. Reynolds: U.S. Patent 7,064,108 2006, to Merck Co[18] Curdová E, Jechová V, Zima J et al. J. Basic Microbiol, 1989, 29: 341-346[19] Waldstein E. Enzymology of Nucleotide Excision Repair. In: Hanawalt PC, Friedberg EC. 1978[20] Sancar A, Rupp W D. Cell, 1983, 33: 249-260[21] Domiflski Z, Jachymczyk W J. Gen Genet, 1984, 193: 167-171[22] ZHU C H, HE Y N, LU F P et al. Nuclear Techniques, 2006, 29(8): 609-613 (in Chinese)[23] Tzuhien V W, Kendric C. Smith. Mol. Gen Genet, 1985, 201: 186-191
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WANG Shu-Yang, CHEN Ji-Hong, LI Wen-Jian, LIANG Jian-Ping, BO Yong-Heng, MA Xiao-Qi and LIU Jing. Influence of 12C6+ ion irradiation on mutant avermitilis[J]. Chinese Physics C, 2012, 36(11): 1140-1144. doi: 10.1088/1674-1137/36/11/019
WANG Shu-Yang, CHEN Ji-Hong, LI Wen-Jian, LIANG Jian-Ping, BO Yong-Heng, MA Xiao-Qi and LIU Jing. Influence of 12C6+ ion irradiation on mutant avermitilis[J]. Chinese Physics C, 2012, 36(11): 1140-1144.  doi: 10.1088/1674-1137/36/11/019 shu
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Received: 2011-09-20
Revised: 1900-01-01
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Influence of 12C6+ ion irradiation on mutant avermitilis

    Corresponding author: CHEN Ji-Hong,
    Corresponding author: LI Wen-Jian,

Abstract: The effects of 12C+6 ion irradiation on colony morphology and mycelia morphology, as well as on mutation rate have been studied in the B1a high-product strains (ZJAV-Y1-203) mutated by heavy ion irradiation and compared with that in the original strain (ZJAV-A-1). After irradiating the rate of a straw hat colony type having a high ability of producing B1a in ZJAV-Y1-203 strains was higher than that found in ZJAV-A-1 strains. When strains were cultured in a liquid medium for 24 hours, the mycelium becoming thinner could be observed in all of the irradiated ZJAV- Y1-203 groups, but only in the ZJAV-A-1 groups irradiated at the dose of 50 Gy or more. The early growth of mycelium was inhibited in the ZJAV- Y1-203 group irradiated with a high dose. The highest positive mutation rate (23.5%) of ZJAV - Y1 - 203 was reached at the lower dose of 30 Gy while the highest positive mutation rate of 34.2% in ZJAV-A-1 appeared at 50 Gy.
These results indicate that the effects of heavy ion irradiation still exist even in the mutated Streptomyces avermitilis, and only the dose is lower and the effects not so strong compared with the one that is first irradiated with optimized heavy ion doses. This is evidence of the one directional mutation being controlled by many more factors in a organism.

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